Taxonomic Significance of Trichome Ultrastructure in Five Genera of Lamiaceae


  • Norhazila Hussin Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim Perak, Malaysia
  • Nor Nafizah Mohd Noor Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim Perak, Malaysia
  • Fatimah Mohamed Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim Perak, Malaysia



SEM, Trichomes, Glandular, Non-glandular, Lamiaceae, Ultrastructure


The trichome of 12 species from five problematic genera of the Lamiaceae family, comprising Petraeovitex bambusetorum, Petraeovitex membranacea, Petraeovitex wolfei, Petraeovitex scortechinii, Petraeovitex trifoliata, Peronema canescens, Congea forbesii, Congea griffithiana, Sphenodesme racemosa, Sphenodesme triflora, Sphenodesme pentandra, and Rotheca serrata was investigated under scanning electron microscope (SEM). This study aimed to assess their ultrastructure and ascertain their respective taxonomic value accordingly. Two types of glandular trichomes, specifically peltate and capitate, and three types of non-glandular trichomes, namely multicellular uniseriate, stellate, and conical were thus observed. The stellate type of non-glandular trichome was solely found in S. pentandra among the 12 studied species, while the data further demonstrated both Congea species to reflect the multicellular uniseriate structure on the respective leaf surfaces, whereas C. forbesii depicted the longest length size among others at 600 µm. In the case of P. canescens and R. serrata, both were found to be with compact trichomes on both surfaces. Going forward, new data on trichome types, distribution, and size, particularly of these 12 studied species, would provide useful guidance for infrageneric classification.


Download data is not yet available.


Agustin, Y. T., Ermayanti, E., & Susanti, R. (2022). Leaf trichomes identification in Lamiaceae family plants and contribution to high school biology learning. JPBIO (Jurnal Pendidikan Biologi), 7(1), 20-35.

Amran, A. N., Noor, N. N. M., Mohamed, F (2023). Micromorphological analyses of stomata and epidermal cell among different species in the genus Premna L. (Lamiaceae). Malaysian Journal Of Microscopy, 19(1), 319-332.

Ascensao, L., & Pais, M. S. (1998). The leaf capitate trichomes of Leonotis leonurus: histochemistry, ultrastructure and secretion. Annals of Botany, 81(2), 263-271.

Ascensao, L.., Mota L. and Castro, M. D. M. (1999). Glandular trichomes on the leaves and flowers of Plectranthus Ornatus: Morphology, distribution and histochemistry. Annals of Botany, 84, 437-447.

Cantino, P. D. (1990). The phylogenetic significance of stomata and trichomes in the Labiatae and Verbenaceae. Journal of the Arnold Arboretum, 71, 323-370.

Choi, J. S., & Kim, E. S. (2013). Structural features of glandular and non-glandular trichomes in three species of Mentha. Applied Microscopy, 43(2), 47-53.

Dmitruk, M., & Weryszko-Chmielewska, E. (2010). Morphological differentiation and distribution of non-glandular and glandular trichomes on Dracocephalum moldavicum L. shoots. Acta agrobotanica, 63(1), 11-22.

Freitas, D. M., Reis, A., L da Costa Bortoluzzi, R., & Santos, M. (2014). Morphological and micromorphological characteristics of Desmodium fruits (Leguminosae: Papilionoideae). Revista de biologia tropical, 62(4), 1597-1608.

Giuliani, C. & Bini, L.M. (2008). Insight into the structure and chemistry of glandular trichomes of Labiatae, with emphasis on subfamily Lamioideae. Plant System Evolution. 276, 199–208.

Giuliani, C., Pellegrino, R., Tirillini, B., & Bini, L. M. (2008). Micromorphological and chemical characterisation of Stachys recta L. subsp. serpentini (Fiori) Arrigoni in comparison to Stachys recta L. subsp. recta (Lamiaceae). Flora-Morphology, Distribution, Functional Ecology of Plants, 203(5), 376-385.

Jia, P., Liu, H., Gao, T., & Xin, H. (2013). Glandular trichomes and essential oil of Thymus quinquecostatus. The Scientific World Journal, 2013,1-8.

Kahraman, A., Celep, F., & Dogan, M. (2010). Anatomy, trichome morphology and palynology of Salvia chrysophylla Stapf (Lamiaceae). South African Journal of Botany, 76(2), 187-195.

Kaya, A., Demirci, B., & Baser, K. H. C. (2007). Micromorphology of glandular trichomes of Nepeta congesta Fisch. & Mey. var. congesta (Lamiaceae) and chemical analysis of the essential oils. South African Journal of Botany, 73(1), 29-34.

Mannethody, S., & Purayidathkandy, S. (2018). Trichome micromorphology and its systematic significance in Asian Leucas (Lamiaceae). Flora, 242, 70-78.

Manan, A. A., Taha, R. M., Mubarak, E. E., & Elias, H. (2016). In vitro flowering, glandular trichomes ultrastructure, and essential oil accumulation in micro propagated Ocimum basilicum L. In Vitro Cellular & Developmental Biology-Plant, 52(3), 303-314.

Mauseth, J. D. (1998). Plant anatomy: California: Benjamin/Cummings Publishing Company.

Metcalfe, C.R. & Chalk, L. (1950) Anatomy of the Dicotyledons. Vol. 1. Oxford: Clarendon Press.

Noor, N. N. M., Mohamed, F., Daud, N., Alimon, H., Rahman, M. A., & Emak, H. A. (2018). Comparative anatomical study and morphological observation of three medicinal Vitex species (Lamiaceae). Malayan Nature Journal, 70(3), 285-296.

Payne, W. W. (1978). A glossary of plant hair terminology. Brittonia, 30(2), 239-255.

Phumprasert, F. (2018). Comparative leaf blade anatomy and genetic relationship by iPBS Marker of Clerodendrum L., Rotheca Raf. and Volkameria L.(Lamiaceae) in Thailand. Doctoral dissertation. Prince of Songkla University.

Raman, V., Lata, H., Chandra, S., Khan, I. A., & ElSohly, M. A. (2017). Morpho-anatomy of Marijuana (Cannabis sativa L.). In Cannabis sativa L.-Botany and Biotechnology. New York: Springer, Cham.

Salmaki, Y., Zarre, S., Jamzad, Z., & Bräuchler, C. (2009). Trichome micromorphology of Iranian Stachys (Lamiaceae) with emphasis on its systematic implication. Flora-Morphology, Distribution, Functional Ecology of Plants, 204(5), 371-381.

Satil, F., & Kaya, A. (2007). Leaf anatomy and hairs of Turkish Satureja L. (Lamiaceae). Acta Biologica Cracoviensia Series Botanica, 49(1), 67-76.

Simpson, M. G. (2019). Plant systematics. Elsevier-Academic Press.

Werker, E. (2000). Trichome diversity and development. Advances in Botanical Research, 31, 1-35.

Werker, E., Putievsky, E., Ravid, U., Dudai, N., & Katzir, I. (1993). Glandular hairs and essential oil in developing leaves of Ocimum basilicum L.(Lamiaceae). Annals of Botany, 71(1), 43-50.

Zhao, F., Chen, Y. P., Salmaki, Y., Drew, B. T., Wilson, T. C., Scheen, A. C., & Xiang, C. L. (2021). An updated tribal classification of Lamiaceae based on plastome phylogenomics. BMC biology, 19(2), 1-27.

Zin, S. M., Mohamed, F., & Noor, N. N. M. (2022a). A comprehensive trichome investigation of Vitex L.(Lamiaceae martinov) in Peninsular Malaysia and its taxonomic significance. Malaysian Journal of Microscopy, 18(2), 92-107.

Zin, S. M., Mohamed, F., & Noor, N. N. M. (2022b). Numerical taxonomic evaluation of leaf architectural morphology of Vitex L. species (Lamiaceae Martinov) in Peninsular Malaysia. Journal of Science and Mathematics Letters, 10(2), 1-15.




How to Cite

Hussin, N., Mohd Noor, N. N., & Mohamed, F. (2024). Taxonomic Significance of Trichome Ultrastructure in Five Genera of Lamiaceae. Journal of Science and Mathematics Letters, 12(1), 8–17.

Most read articles by the same author(s)