Ultrasonic Assisted Extraction and Gas Chromatography-Tandem Mass Spectroscopy (GC-MS/MS) Analysis of Extracts from Four Cassia Species


  • Saidu Jibril Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, Gombe, Nigeria
  • Hasnah Mohd Sirat Faculty of Science, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia
  • Maikudi Musa Idris Faculty of Physical Sciences, Bayero University Kano, BUK, Kano, Nigeria
  • Aminu Mohammad Faculty of Physical Sciences, Bayero University Kano, BUK, Kano, Nigeria
  • Kamal Ja’afar Muhammad Chemistry Advanced Research Centre, Sheda Science and Technology Complex, 186 Garki, Abuja, Nigeria




Cassia, GC-MS/MS, phytoconstituents, extraction, fatty acid


Ultrasonic assisted extraction (UAE) process increases the rate of extraction, the yield of extracted components and reduces extraction time. The high sensitivity of GC-MS/MS provides reliable identification of phytoconstituents present in a plant extract. This research is to develop an easy and effective method for the extraction and analysis of phytochemicals in C. singueana, C. sieberiana, C. tora and C. occidentalis using UAE and GC-MS/MS techniques. The crude extracts obtained from the four Cassia species under UAE conditions of 50 mins extraction time, 50 oC extraction temperature and 20 mL/g solvent to sample ratio were analyzed using GC-MS/MS. Thirty-four compounds were identified by comparison of their mass spectra with those in the National Institute of Standards and Technology library, requiring at least 80% similarity. These compounds comprise fatty acids, fatty alcohol, carboxylic acid, long-chain alkanes, diterpenes, triterpenes, sterols and anthraquinones. n-Hexadecanoic acid and phytol were identified in all the Cassia species while anthraquinone was detected only from the methanol extracts of C. tora and C. occidentalis. The bioactivities reported in the literature for these compounds corroborate with the phytoconstituents identified in these plants and support the ethnomedicinal uses of these Cassia species.


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How to Cite

Jibril, S., Sirat, H. M., Idris, M. M., Mohammad, A., & Muhammad, K. J. (2020). Ultrasonic Assisted Extraction and Gas Chromatography-Tandem Mass Spectroscopy (GC-MS/MS) Analysis of Extracts from Four Cassia Species. Journal of Science and Mathematics Letters, 8(2), 1–7. https://doi.org/10.37134/jsml.vol8.2.1.2020