Isolation and Characterization of Friedelin and 5-DodecylResorcinol from the Stem Bark Extract of Pterocarpus erinaceus

Authors

  • Marwan Ibrahim Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Bayero University Kano, P.M.B. 3011, Kano, NIGERIA
  • Musa Maikudi Idris Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Bayero University Kano, P.M.B. 3011, Kano, NIGERIA
  • Usman Dauda Department of Chemistry, Faculty of Science, Taraba State University, PMB 1167, Jalingo, Taraba, NIGERIA
  • Umar Ali Department of Chemistry, Faculty of Natural and Applied Sciences, Sule Lamido University, Kafin Hausa, Jigawa, NIGERIA
  • Aminu Muhammad Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Bayero University Kano, P.M.B. 3011, Kano, NIGERIA

DOI:

https://doi.org/10.37134/jsml.vol10.2.9.2022

Keywords:

Pterocarpus erinaceus, friedelin, resorcinol, cytotoxicity, microbes

Abstract

Pterocarpus erinaceus (Fabaceae) is a medicinal plant used in the treatment of several diseases such as, fever, diarrhea, and dysentery. The aim of this research work is to isolate phytochemicals, evaluate antimicrobial and cytotoxic activities of the extract/isolated compounds from the stem bark of Pterocarpus erinaceus.  The plant material was air dried and extracted with a polarity gradient of petroleum ether, ethyl acetate, and methanol.  Purification of the methanol extract using column chromatography yielded two compounds: friedelin (1) and 5-dodecylresorcinol (2). Antimicrobial screening of the extracts and isolated compounds was conducted using the disc diffusion method, while the cytotoxicity was tested on the extracts using Brine Shrimp larvae. The methanol extract was more toxic (LC50 of 0.93 µg/mL) than petroleum ether (PER-PE) and ethyl acetate (PER-EA) extracts. Antimicrobial screening of the plant extracts and compounds revealed a moderate activity at 1000 µg/mL.

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References

Amos, S., Okwuasaba, F.K., Gamaniel, K., Akah, P., & Wambebe, C. (1998). Inhibitory effects of the aqueous extract of Pavetta crissipes leaves on gastrointestinal and uterine smooth muscle preparations isolated from rabbits, guinea pig and rats. Journal of Ethnopharmacology, 61, 209-213.

Benzie, I.F.F. & Wachtel-Galor, S. (2011). Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Boca Raton (FL): CRC Press Taylor & Francis.

Chikezie, P.C., Ibegbulem, C.O., & Mbagwu, F.N. (2015). Bioactive principles from medicinal plants. Research Journal of Phytochemistry, 9(3), 88-115.

Dawurung, C.J., Nguyen M.T.H., Pengon, J., Dokladda, K., Bunyong, R., Rattanajak, R., Kamchonwongpaisan, S., Nguyen, P.T.M., & Pyne, S.G. (2021). Isolation of bioactive compounds from medicinal plants used in traditional medicine: Rautandiol B, a potential lead compound against Plasmodium falciparum. BMC Complementary Medicine and Therapies, 21, 1-12.

Hutchinson, J. & Dalziel, J.M. (1958). Flora of west tropical Africa. In: Keay, R.W.J., Ed., 2nd Edition,Vol. 1, part 2, published on Behalf of the Governments of Nigeria, Ghana, Sierra Leone & The Gambia by Crown Agents for Overseas Governments and Administrations, London.

Karaman, I., Sahin, F., Gulluce, M., Ogutçu, H., Sengul, M., & Adiguzel, A. (2003) Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. Journal of Ethnopharmacology, 85, 231-235.

Megawati, A.D. (2017) Resorcinol Compounds Isolated form the Bark of Myristica fatua Houtt. Indonesian Journal of Pharmacy, 28, 82-90.

Muhammad, A. & Sirat, H.M. (2013): Antimicrobial, antityrosinase and brine shrimp lethality test of Bauhinia rufescens Lam (Fabaceae). Journal of Coastal Life Medicine, 1(2), 123-128.

Odeh, I.C., Tor-Anyiin, T.A., Igoli, J.O., & Anyam, J.V. (2016). In vitro antimicrobial properties of friedelan-3-one from Pterocarpus santalinoides Lam. African Journal of Biotechnology, 15(14), 531-538.

Queiroga, C.L., Silva, G.F., Dias, P.C., Possenti, A., & de Carvalho, J.E. (2000). Evaluation of the antiulcerogenic activity of friedelan-3β-ol and friedelin isolated from Maytenus ilicifolia (Celastraceae). Journal of Ethnopharmacology, 72(3), 465-468.

Sandrine, P. (2006). Pterocarpus erinaceus;State of Forest Genetic Resources in the Sahelian and Northern Sudanian Zone of Africa (FAO). Trease G.E. and Evans W.C. (1983). Textbook of Pharmacognosy 12thEdition, Balliere Tindal, London pp14-25.

Shahinozzaman, M., Ishii, T., Halim, M.A., Hossain, M.A., Islam, M.T., & Tawata, S. (2019). Cytotoxic and anti-inflammatory resorcinol and alkylbenzoquinone derivatives from the leaves of Ardisia sieboldii. Zeitschrift für Naturforschung C – Journal of Bioscience, 74(11-12), 303-311.

Syahmi, A.R.M., Vijayarathna, S., Sasidharan, S., Latha, L.Y., Kwan, Y.P., Lau, Y.L., Shin, L.N., & Chen, Y. (2010). Acute oral toxicity and brine shrimp lethality of Elaeis guineensis Jacq., (Oil Palm Leaf) methanol extract. Molecules, 15, 8111-8121.

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Published

2022-10-15

How to Cite

Ibrahim, M., Idris, M. M., Dauda, U., Ali, U., & Muhammad, A. (2022). Isolation and Characterization of Friedelin and 5-DodecylResorcinol from the Stem Bark Extract of Pterocarpus erinaceus. Journal of Science and Mathematics Letters, 10(2), 74–80. https://doi.org/10.37134/jsml.vol10.2.9.2022

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