Resolvability Between Bare and DNA-Grafted Microsphere by Flow Resistance Measurement using Optical Tweezers
Kebolehbezaan antara Mikrosfera Tanpa Salut dan Mikrosfera Bersalut DNA dengan Kaedah Pengukuran Rintangan Alir Menggunakan Penyepit Optik
Keywords:
optical tweezers, flow-resistance, DNAAbstract
Optical tweezers have an ability to hold a single optically transparent particle in a suitable medium without mechanical contact. One can measure and exert photonics forces of focused laser power on a particle in confined test cell. They offer a possibility to hold a particle in a flow of surrounding medium. For a bare micro sphere, Stoke’s law easily holds. But for 4000-bp DNA grafted micro sphere, the flow resistance is unknown and theoretically difficult to describe. Experimentally, the optical tweezers resolve between bare and the grafted microsphere in phosphate buffer solution (PBS) pH 7.4 by 2.00 ± 0.05 pN at the position of 35 μm from the test cell wall regardless on employed laser power.
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References
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Ven, T. G. M. (1989). Colloidal hydrodynamics. London [etc.]: Academic Press.
Perkins, T. T., Smith, D. E., & Chu, S. (1997). Single polymer dynamics in an elongational flow. Science, 276(5321), 2016-2021.
Ashkin, A. (1997). Optical trapping and manipulation of neutral particles using lasers. Proceedings of the National Academy of Sciences, 94(10), 4853-4860.
Bishop, A. I., Nieminen, T. A., Heckenberg, N. R., & Rubinsztein-Dunlop, H. (2004). Optical Microrheology Using Rotating Laser-Trapped Particles. Physical Review Letters, 92(19), 198104.
Valentine, M. T., Dewalt, L. E., & Ou-Yang, H. D. (1996). Forces on a colloidal particle in a polymer solution: a study using optical tweezers. Journal of Physics: Condensed Matter, 8(47), 94779482.
Sun, S. F. (1884). Physical chemistry of macromolecules: Basic principles and issues. New York: Wiley & Sons.
Rzehak, R., Kromen, W., Kawakatsu, T., & Zimmermann, W. (2000). Deformation of a tethered polymer in uniform flow. The European Physical Journal E, 2(1), 3-30.
Perkins, T. T., Smith, D. E., & Chu, S. (1994). Direct observation of tube-like motion of a single polymer chain. Science, 264(5160), 819-822.
Rajkumar, A. S., & Ali, B. J. (2008). Microrheology of concentrated DNA solutions using optical tweezers. Bulletin of Materials Science, 31(3), 381-386.
Ven, T. G. M. (1989). Colloidal hydrodynamics. London [etc.]: Academic Press.
Perkins, T. T., Smith, D. E., & Chu, S. (1997). Single polymer dynamics in an elongational flow. Science, 276(5321), 2016-2021.
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Published
2013-12-18
How to Cite
Wei, D. C. B., Ayop, S. K., & Kremer, F. (2013). Resolvability Between Bare and DNA-Grafted Microsphere by Flow Resistance Measurement using Optical Tweezers: Kebolehbezaan antara Mikrosfera Tanpa Salut dan Mikrosfera Bersalut DNA dengan Kaedah Pengukuran Rintangan Alir Menggunakan Penyepit Optik. Journal of Science and Mathematics Letters, 1, 28–36. Retrieved from https://ojs.upsi.edu.my/index.php/JSML/article/view/4304
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