Alteration of Fatty Acid Compositions in A Starch-deficient Mutant Arabidopsis thaliana (adg1-1) during Seed Development

Authors

  • Hasimah Alimon
  • W Paul Quick
  • Richard C Leegood

Keywords:

starchless mutant, ADP glucose pyrophosphorylase (ADG), carbon partitioning, seed development, metabolism

Abstract

Disruption of the ADG gene in Arabidopsis results in 6% increase of total fatty acids in mature desiccated seeds. A starchless mutant of Arabidopsis thaliana, ADP glucose pyrophosphorylase (adg1-1) has been investigated concerning the lack of starch in its leaves. In this work of metabolic analysis of adg1-1 mutant, there was very little or no starch accumulation throughout seed development. Even though the growth of these mutant plants was delayed, seed development of the mutants did not show much difference from the wild type. In mature desiccated seeds, there was no significant difference in the seed dry weight of the mutant compared to the wild type. The adg1-1 mutant showed a shift in carbon partitioning to fatty acid (FA) and sucrose accumulation. Major changes have occurred in FA 18.0 (stearic), FA 18.1 (oleic) and FA 18.2 (linoleic). In mature seeds of adg1-1, there was a 70% increase in FA 18.2, a 75% increase in FA 18.1 and a 76% reduction in FA 18.0. The results show that the assimilate partitioning within Arabidopsis seeds has the potential to be altered by relatively simple genetic manipulations. However, the mechanisms by which these changes occurred remain unknown.

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Author Biographies

Hasimah Alimon

Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900, Tg. Malim, Perak, Malaysia

W Paul Quick

Department of Animal and Plant Science, University of Sheffield, United Kingdom

Richard C Leegood

Department of Animal and Plant Science, University of Sheffield, United Kingdom

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Published

2010-12-01

How to Cite

Alimon, H., Quick, W. P., & C Leegood, R. (2010). Alteration of Fatty Acid Compositions in A Starch-deficient Mutant Arabidopsis thaliana (adg1-1) during Seed Development. Journal of Science and Mathematics Letters, 2(2), 18–28. Retrieved from https://ojs.upsi.edu.my/index.php/JSML/article/view/430